Safety Data Sheet
According to Federal Register / Vol. 77, No. 58 / Monday, March 26, 2012 / Rules and Regulations
Product identifier: F1-X™ Next-Generation 1-Step Gibson Assembly® Master Mix
Other means of identification
Product codes: F1XGA, F1XMM (product codes cover multiple kit sizes)
Recommended use: Research use only.
Recommended restrictions: None known.
Manufacturer/Importer/Supplier/Distributor information
Company Name: Racer Biosciences
Address: 6868 Nancy Ridge Dr., San Diego, CA 92121-2993, USA
Telephone: 1+858-366-3335
Emergency telephone number: 1+858-366-3335
E-mail: info@racerbio.com
Contact Person: Customer Service
Physical hazards: Not classified.
Health hazards: Not classified.
OSHA defined hazards: Not classified.
Label elements
Hazard symbol: None
Signal word: None
Hazard statement: The mixture does not meet the criteria for classification.
Precautionary statement
Prevention: Observe good industrial hygiene practices.
Response: Wash hands after handling.
Storage: Store away from incompatible materials.
Disposal: Dispose of waste and residues in accordance with local authority requirements.
Hazard(s) not otherwise classified (HNOC): None.
Supplemental information: None.
Materials
| Chemical Name | CAS Number | Concentration* |
|---|---|---|
| Glycerol | 56-81-5 | ≥15 – ≤25% |
*All concentrations are in percent by weight unless ingredient is a gas. Gas concentrations are in percent by volume.
Inhalation: Move to fresh air. Call a physician if symptoms develop or persist.
Skin contact: Wash off with soap and water. Get medical attention if irritation develops and persists.
Eye contact: Rinse with water. Get medical attention if irritation develops and persists.
Ingestion: Rinse mouth. Get medical attention if symptoms occur.
Most important symptoms/effects, acute and delayed: Direct contact with eyes may cause temporary irritation.
Indication of immediate medical attention and special treatment needed: Treat symptomatically.
General information: Ensure that medical personnel are aware of the material(s) involved, and take precautions to protect themselves.
Suitable extinguishing media: Water fog. Foam. Dry chemical powder. Carbon dioxide (CO₂).
Unsuitable extinguishing media: Do not use water jet as an extinguisher, as this will spread the fire.
Specific hazards arising from the chemical: During fire, gases hazardous to health may be formed.
Special protective equipment and precautions for firefighters: Self-contained breathing apparatus and full protective clothing must be worn in case of fire.
Fire fighting equipment/instructions: Move containers from fire area if you can do so without risk.
Specific methods: Use standard firefighting procedures and consider the hazards of other involved materials.
General fire hazards: No unusual fire or explosion hazards noted.
Personal precautions, protective equipment and emergency procedures: Keep unnecessary personnel away. For personal protection, see Section 8 of the SDS.
Methods and materials for containment and cleaning up
Large Spills: Stop the flow of material, if this is without risk. Dike the spilled material, where this is possible. Cover with plastic sheet to prevent spreading. Absorb in vermiculite, dry sand or earth and place into containers. Following product recovery, flush area with water.
Small Spills: Wipe up with absorbent material (e.g. cloth, fleece). Clean surface thoroughly to remove residual contamination.
Never return spills to original containers for re-use. For waste disposal, see Section 13 of the SDS.
Environmental precautions: Avoid discharge into drains, water courses or onto the ground.
Precautions for safe handling: Avoid prolonged exposure. Observe good industrial hygiene practices.
Conditions for safe storage, including any incompatibilities: Store in original tightly closed container. Store away from incompatible materials (see Section 10 of the SDS).
Ingredients with workplace control parameters
| Components | CAS-No. | Value Type (Form of exposure) | Control Parameters | Basis |
|---|---|---|---|---|
| Glycerol | 56-81-5 | TWA (Mist, total dust) | 15 mg/m³ | OSHA Z-1 |
| Glycerol | 56-81-5 | TWA (Mist, respirable fraction) | 5 mg/m³ | OSHA Z-1 |
Biological limit values: No biological exposure limits noted for the ingredient(s).
Appropriate engineering controls: Good general ventilation (typically 10 air changes per hour) should be used. Ventilation rates should be matched to conditions. If applicable, use process enclosures, local exhaust ventilation, or other engineering controls to maintain airborne levels below recommended exposure limits. If exposure limits have not been established, maintain airborne levels to an acceptable level.
Individual protection measures, such as personal protective equipment
Eye/face protection: Wear safety glasses with side shields (or goggles).
Skin protection: Wear appropriate chemical resistant gloves.
Hand protection: Suitable gloves can be recommended by the glove supplier.
Other: Wear suitable protective clothing.
Respiratory protection: In case of insufficient ventilation, wear suitable respiratory equipment.
Thermal hazards: Wear appropriate thermal protective clothing, when necessary.
General hygiene considerations: Always observe good personal hygiene measures, such as washing after handling the material and before eating, drinking, and/or smoking. Routinely wash work clothing and protective equipment to remove contaminants.
Appearance
Physical state: Liquid.
Form: Liquid.
Color: Colorless.
Odor: Slight chemical.
Odor threshold: Not available.
pH: 7 – 9
Melting point/freezing point: Not available.
Initial boiling point and boiling range: Not available.
Flash point: Not available.
Evaporation rate: Not available.
Flammability (solid, gas): Not available.
Upper/lower flammability or explosive limits: Not available.
Flammability – lower (%): Not available
Flammability – upper (%): Not available
Explosive limit – lower (%): Not available
Explosive limit – upper (%): Not available
Vapor pressure: Not available.
Vapor density: Not available.
Relative density: Not available.
Solubility(ies):
Solubility (water): 100%
Solubility temp. (water): 77 °F (25 °C)
Partition coefficient (n-octanol/water): Not available.
Auto-ignition temperature: Not available.
Decomposition temperature: Not available.
Viscosity: Not available.
Other information
Explosive properties: Not explosive.
Oxidizing properties: Not oxidizing.
Reactivity: The product is stable and non-reactive under normal conditions of use, storage, and transport.
Chemical stability: Material is stable under normal conditions.
Possibility of hazardous reactions: No dangerous reaction known under normal conditions of use, storage and transport.
Conditions to avoid: Contact with incompatible materials.
Incompatible materials: Strong oxidizing agents.
Hazardous decomposition products: No hazardous decomposition products are known.
Information on likely routes of exposure
Inhalation: Prolonged inhalation may be harmful.
Skin contact: No adverse effects due to skin contact are expected.
Eye contact: Direct contact with eyes may cause temporary irritation.
Ingestion: Expected to be a low ingestion hazard.
Symptoms related to the physical, chemical and toxicological characteristics: Direct contact with eyes may cause temporary irritation.
Information on toxicological effects
Acute toxicity: Not expected to be acutely toxic.
Components – Glycerol (CAS 56-81-5)
| Route | Species | Test Results* |
|---|---|---|
| Dermal LD50 | Guinea pig | 56,750 mg/kg |
| Inhalation LC50 | Rat | 4 h – > 5,850 mg/l (aerosol) |
| Oral LD50 | Rat | 27,200 mg/kg |
*Values represent data for pure glycerol.
Skin corrosion/irritation: Not listed.
Serious eye damage/eye irritation: Direct contact with eyes may cause temporary irritation.
Respiratory or skin sensitization: Prolonged skin contact may cause temporary irritation.
Respiratory sensitization: Not a respiratory sensitizer.
Skin sensitization: This product is not expected to cause skin sensitization.
Germ cell mutagenicity: No data available to indicate product or any components present at greater than 0.1% are mutagenic or genotoxic.
Carcinogenicity: This product is not considered to be a carcinogen by IARC, ACGIH, NTP, or OSHA.
IARC Monographs – Overall Evaluation of Carcinogenicity: Not listed.
NTP Report on Carcinogens: Not listed.
OSHA Specifically Regulated Substances (29 CFR 1910.1001-1050): Not regulated.
Reproductive toxicity: This product is not expected to cause reproductive or developmental effects.
Specific target organ toxicity – single exposure: Not classified.
Specific target organ toxicity – repeated exposure: Not classified.
Aspiration hazard: Not an aspiration hazard.
Chronic effects: Prolonged inhalation may be harmful.
Ecotoxicity: The product is not classified as environmentally hazardous. However, this does not exclude the possibility that large or frequent spills can have a harmful or damaging effect on the environment.
Persistence and degradability: No data is available on the degradability of this product.
Bioaccumulative potential: Not available.
Mobility in soil: The product is soluble in water.
Other adverse effects: No other adverse environmental effects (e.g. ozone depletion, photochemical ozone creation potential, endocrine disruption, global warming potential) are expected from this component.
Disposal instructions: Collect and reclaim or dispose in sealed containers at licensed waste disposal site.
Local disposal regulations: Dispose in accordance with all applicable regulations.
Hazardous waste code: The waste code should be assigned in discussion between the user, the producer and the waste disposal company.
Waste from residues/unused products: Dispose of in accordance with local regulations. Empty containers or liners may retain some product residues. This material and its container must be disposed of in a safe manner.
Contaminated packaging: Since emptied containers may retain product residue, follow label warnings even after container is emptied. Empty containers should be taken to an approved waste handling site for recycling or disposal.
DOT: Not regulated as dangerous goods.
IATA: Not regulated as dangerous goods.
IMDG: Not regulated as dangerous goods.
Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code: Not established.
US federal regulations: This product is not known to be a “Hazardous Chemical” as defined by the OSHA Hazard Communication Standard, 29 CFR 1910.1200.
TSCA Section 12(b) Export Notification (40 CFR 707, Subpt. D): Not regulated.
OSHA Specifically Regulated Substances (29 CFR 1910.1001-1050): Not regulated.
CERCLA Hazardous Substance List (40 CFR 302.4): Not regulated.
Superfund Amendments and Reauthorization Act of 1986 (SARA)
Hazard categories:
Immediate Hazard – No | Delayed Hazard – No | Fire Hazard – No | Pressure Hazard – No | Reactivity Hazard – No
SARA 302 Extremely hazardous substance: Not listed.
SARA 311/312 Hazardous chemical: No
SARA 313 (TRI reporting): Not regulated.
Other federal regulations
Clean Air Act (CAA) Section 112 Hazardous Air Pollutants (HAPs) List: Not regulated.
Clean Air Act (CAA) Section 112(r) Accidental Release Prevention (40 CFR 68.130): Not regulated.
Safe Drinking Water Act (SDWA): Not regulated.
US state regulations
US. Massachusetts RTK – Substance List: Not regulated.
US. New Jersey Worker and Community Right-to-Know Act: Not listed.
US. Pennsylvania Worker and Community Right-to-Know Law: Not listed.
US. Rhode Island RTK: Not regulated.
US. California Proposition 65: California Safe Drinking Water and Toxic Enforcement Act of 1986 (Proposition 65): This material is not known to contain any chemicals currently listed as carcinogens or reproductive toxins.
International Inventories
| Inventory | Status |
|---|---|
| TSCA | Contact supplier for inventory compliance status. |
| DSL/NDSL | Contact supplier for inventory compliance status. |
| EINECS/ELINCS | Contact supplier for inventory compliance status. |
| ENCS | Contact supplier for inventory compliance status. |
| IECSC | Contact supplier for inventory compliance status. |
| KECL | Contact supplier for inventory compliance status. |
| PICCS | Contact supplier for inventory compliance status. |
| AICS | Contact supplier for inventory compliance status. |
| NZIoC | Contact supplier for inventory compliance status. |
Legend: TSCA – US Toxic Substances Control Act Section 8(b) Inventory; DSL/NDSL – Canadian Domestic/Non-Domestic Substances List; EINECS/ELINCS – European Inventory of Existing/Notified Chemical Substances; ENCS – Japan Existing and New Chemical Substances; IECSC – China Inventory of Existing Chemical Substances; KECL – Korean Existing and Evaluated Chemical Substances; PICCS – Philippines Inventory of Chemicals and Chemical Substances; AICS – Australian Inventory of Chemical Substances; NZIoC – New Zealand Inventory of Chemicals
Issue date: May 5, 2016
Revision date: March 3, 2026
Version: 4
List of Abbreviations
DOT: Department of Transportation. IATA: International Air Transport Association. IMDG: International Maritime Dangerous Goods. TWA: Time Weighted Average. LD50: Lethal dose 50%. LC50: Lethal concentration 50%.
Revision information
Updated product name. Clarifications made to section 11.